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KMID : 0380019950100010046
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 1 p.46 ~ p.54
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Optimal Nutritional Requirements of Carrot Hairy Roots
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Kim Ji-Hyeon
Yoo Young-Je
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Abstract
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The physiological characteristics of carrot hairy roots and suspension cells were examined based on their nutritional requirements. Inorganic nutrient (phosphorous and ammonium) requirements of carrot hairy roots were similar to those of suspension cells. Optimal sucrose concentration for the growth of hairy roots (7%) was different from that of suspension cells (3%). Since suspension cells were more easily affected by the environmental condition, e.g., osmotic stress, than hairy roots which made the suitable growth condition for cells, it can be understood that optimal sucrose concentration for the growth of hairy roots was higher than that for the growth of the suspension cells. To investigate the roles of sucrose on the growth of hairy roots, the effects of sucrose on the fresh weight and dry weight was analysed by the addition of mannitol as an osmoticum. Sucrose acts also as an energy source for hairy roots rather than as an osmotic regulato;since the increase of dry weight was higher than that of fresh weight at the given sucrose concentration.
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